Journal
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
Volume 69, Issue 5, Pages 527-531Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/gerona/glt121
Keywords
Leukocyte telomere length; Quantitative PCR; Southern blots
Categories
Funding
- Netherlands Organization for Scientific Research (NWO)
- NIH [R01AG030678, R01HD071180]
Ask authors/readers for more resources
Only a few studies, primarily limited to small samples, have examined the relationship between leukocyte telomere length (LTL) data generated by Southern blots, expressed in kilobases, versus quantitative PCR data, expressed in the telomere product/a single gene product (T/S). In the present study, we compared LTL data generated by the two methods in 681 elderly participants (50% African Americans, 50% of European origin, 49.2% women, mean age 73.72.9 years) in the Health Aging and Body Composition Study. The correlation between the data generated by the two methods was modest (R-2 .27). Both methods captured the age effect on LTL and the longer LTL in women than in men. However, only the Southern blot method showed a significantly longer LTL in African Americans than in European decent individuals, which might be attributed to the larger measurement error of the quantitative PCRbased method than the Southern blots.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available