Journal
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
Volume 66, Issue 10, Pages 1062-1076Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/gerona/glr080
Keywords
GHRKO mice; Mitochondrial biogenesis; Gene expression; Calorie restriction; Visceral fat removal
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Funding
- National Institute on Aging [AG 19899, U19 AG023122, AG31736, AG032290]
- Ellison Medical Foundation
- Southern Illinois University School of Medicine
- Claude D. Pepper Older Americans Independence Center [1P30AG028740]
- Polish Ministry of Science and Higher Education [N N401 042638]
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Mitochondrial biogenesis is essential for cell viability. Growth hormone receptor knockout (GHRKO), calorie restriction, and surgical visceral fat removal constitute experimental interventions to delay aging and increase life span. We examined the expression of known regulators of mitochondriogenesis: peroxisome proliferator-activated receptor gamma co-activator 1 alpha (PGC-1 alpha), adenosine monophosphate (AMP)-activated protein kinase (AMPK), sirtuin-1 (SIRT-1) and sirtuin-3 (SIRT-3), endothelial nitric oxide synthase (eNOS), nuclear respiratory factor-1, mitochondrial transcription factor A (TFAM), and mitofusin-2 (MFN-2) in the skeletal muscles and hearts of control and calorie-restricted female GHRKO mice and in the kidneys of male GHRKOs after visceral fat removal or sham surgery. Expression of PGC-1 alpha in skeletal muscles, AMPK, SIRT-1, SIRT-3, eNOS, and MFN-2 in the heart and PGC-1 alpha, AMPK, SIRT-3, eNOS, and MFN-2 in kidneys was increased in GHRKO mice but was not affected by calorie restriction or visceral fat removal. GHRKO mice have increased expression of key regulators of mitochondriogenesis, which is not improved further by calorie restriction or visceral fat removal.
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