Journal
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
Volume 12, Issue 11, Pages 931-934Publisher
ZHEJIANG UNIV
DOI: 10.1631/jzus.B1100065
Keywords
Glucosamine; alpha-Amylase; Glucoamylase; Hydrolysis
Categories
Funding
- Jiangsu Key Laboratory of Marine Biotechnology, China [2009HS07]
Ask authors/readers for more resources
In order to overcome the defects of chemical hydrolysis approach to prepare glucosamine, an enzymatic hydrolysis method was developed. Glucosamine was prepared by hydrolyzing chitosan, employing alpha-amylase initially, and subsequently, glucoamylase. The optimal hydrolyzing conditions were as follows: reaction time, 4 h; pH, 5.0; temperature, 50 A degrees C; and, alpha-amylase, 80 U/g for the initial reaction. Subsequently, glucoamylase was added in the presence of alpha-amylase. The optimal reaction conditions were found to be: reaction time, 8 h; pH, 4.5; temperature, 55 A degrees C; and, glucoamylase, 4 000 U/g. The hydrolysates were subject to filtrating, concentrating to about 20% (w/w), precipitating with five volumes of ethanol, and drying at 60 A degrees C for 2 h. The content and the yield of glucosamine in the dried precipitate were 91.3% (w/w) and 86.2% (w/w), respectively. The method developed in this study is a promising option in the preparation of glucosamine.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available