Journal
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
Volume 11, Issue 7, Pages 524-530Publisher
ZHEJIANG UNIV
DOI: 10.1631/jzus.B0900400
Keywords
Collagen; Chitosan; Fibrin glue; Scaffold; Tissue-engineered skin
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Funding
- National Basic Research Program (973) of China [2005CB623902-1]
- Science Research Foundation of the Ministry of Health of China [WKJ2006-2-2007]
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To create a scaffold that is suitable for the construction of tissue-engineered skin, a novel asymmetric porous scaffold with different pore sizes on either side was prepared by combining a collagen-chitosan porous membrane with fibrin glue. Tissue-engineered skin was fabricated using this asymmetric scaffold, fibroblasts, and a human keratinocyte line (HaCaT). Epidermal cells could be seen growing easily and achieved confluence on the fibrin glue on the upper surface of the scaffold. Scanning electron microscopy showed typical shuttle-like fibroblasts adhering to the wall of the scaffold and fluorescence microscopy showed them growing in the dermal layer of the scaffold. The constructed composite skin substitute had a histological structure similar to that of normal skin tissue after three weeks of culture. The results of our study suggest that the asymmetric scaffold is a promising biologically functional material for skin tissue engineering, with prospects for clinical applications.
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