Journal
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
Volume 11, Issue 10, Pages 801-805Publisher
ZHEJIANG UNIV
DOI: 10.1631/jzus.B1000051
Keywords
Human cells; Chromatin; Heterochromatin; Electromagnetic field; Elliptical polarization
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To investigate the influence of microwave radiation on the human fibroblast nuclei, the effects of three variants of electromagnetic wave polarization, linear and left-handed and right-handed elliptically polarized, were examined. Experimental conditions were: frequency (f) 36.65 GHz, power density (P) at the surface of exposed object 1, 10, 30, and 100 mu W/cm(2), exposure time 10 s. Human fibroblasts growing in a monolayer on a cover slide were exposed to microwave electromagnetic radiation. The layer of medium that covered cells during microwave exposure was about 1 mm thick. Cells were stained immediately after irradiation by 2% (w/v) orcein solution in 45% (w/v) acetic acid. Experiments were made at room temperature (25 A degrees C), and control cell samples were processed in the same conditions. We assessed heterochromatin granule quantity (HGQ) at 600x magnification. Microwave irradiation at the intensity of 1 mu W/cm(2) produced no effect, and irradiation at the intensities of 10 and 100 mu W/cm(2) induced an increase in HGQ. More intense irradiation induced more chromatin condensation. The right-handed elliptically polarized radiation revealed more biological activity than the left-handed polarized one.
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