Journal
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
Volume 11, Issue 11, Pages 862-870Publisher
ZHEJIANG UNIV
DOI: 10.1631/jzus.B1000048
Keywords
Phalaris coerulescens thioredoxin gene (PTrx); Aluminum; Transgenic barley; Oxidative stress
Categories
Funding
- National Natural Science Foundation of China [30871530]
- Science and Technology Transformation Plan of Henan Province [0636000005]
- Chinese Academy of Sciences (CAS)
- CAS/SAFEA
- CAS, China [2009Y2B211]
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A transgenic barley line (LSY-11-1-1) with overexpressed Phalaris coerulescens thioredoxin gene (PTrx) was employed to measure the growth, protein oxidation, cell viability, and antioxidase activity in barley roots during germination on the presence of 2 mmol/L AlCl3 on filter paper. The results show that (1) compared with the non-transgenic barley, LSY-11-1-1 had enhanced root growth, although both were seriously inhibited after AlCl3 treatment; (2) the degree of protein oxidation and loss of cell viability in roots of LSY-11-1-1 were much less than those in roots of non-transgenic barley, as reflected by lower contents of protein carbonyl and Evans blue uptakes in LSY-11-1-1; (3) activities of catalase (CAT), glutathione peroxidase (GPX), ascorbate peroxidase (APX), and glutathione reductase (GR) in LSY-11-1-1 root tips were generally higher than those in non-transgenic barley root tips, although these antioxidase activities gave a rise to different degrees in both LSY-11-1-1 and non-transgenic barley under aluminum stress. These results indicate that overexpressing PTrx could efficiently protect barley roots from oxidative injury by increasing antioxidase activity, thereby quenching ROS caused by AlCl3 during germination. These properties raise the possibility that transgenic barley with overexpressed PTrx may be used to reduce the aluminum toxicity in acid soils.
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