Journal
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
Volume 11, Issue 11, Pages 851-861Publisher
ZHEJIANG UNIV
DOI: 10.1631/jzus.B1000137
Keywords
Brassica compestris L. spp. chinensis; codA; Stress; Glycine betaine; Net photosynthetic rate (P-n)
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Funding
- National Science Foundation of China [30571146]
- National Key Basic Research Special Foundation of China [G1999011700]
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Transgenic Brassica compestris L. spp. chinensis plants expressing a choline oxidase (codA) gene from Arthrobacter globiformis were obtained through Agrobacterium tumefaciens-mediated transformation. In the transgenic plants, codA gene expression and its product transportation to chloroplasts were detected by the enzyme-linked immunosorbent assay (ELISA) examination, immunogold localization, and H-1-nuclear magnetic resonance (H-1-NMR). Stress tolerance was evaluated in the T-3 plants under extreme temperature and salinity conditions. The plants of transgenic line 1 (L1) showed significantly higher net photosynthetic rate (P-n) and P-n recovery rate under high (45 degrees C, 4 h) and low temperature (1 degrees C, 48 h) treatments, and higher photosynthetic rate under high salinity conditions (100, 200, and 300 mmol/L NaCl, respectively) than the wild-type plants. The enhanced tolerance to high temperature and high salinity stresses in transgenic plants is associated with the accumulation of betaine, which is not found in the wild-type plants. Our results indicate that the introduction of codA gene from Arthrobacter globiformis into Brassica compestris L. spp. chinensis could be a potential strategy for improving the plant tolerance to multiple stresses.
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