Journal
PROTEOMICS
Volume 15, Issue 7, Pages 1224-1229Publisher
WILEY
DOI: 10.1002/pmic.201400436
Keywords
Bottom-up proteomics; Comparative proteomics; Gel-assisted sample preparation; Sample preparation; Technology
Funding
- Kennedy Trust for Rheumatology Research
- Medical Research Council, UK
Ask authors/readers for more resources
We describe a gel-assisted proteomic sample preparation method for MS analysis. Solubilized protein extracts or intact cells are copolymerized with acrylamide, facilitating denaturation, reduction, quantitative cysteine alkylation, and matrix formation. Gel-aided sample preparation has been optimized to be highly flexible, scalable, and to allow reproducible sample generation from 50 cells to milligrams of protein extracts. This methodology is fast, sensitive, easy-to-use on a wide range of sample types, and accessible to nonspecialists.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available