Journal
PROTEOMICS
Volume 15, Issue 9, Pages 1598-1607Publisher
WILEY
DOI: 10.1002/pmic.201400083
Keywords
Animal proteomics; Carnivorous; GeLC-MS; MS; Glires; Primates; Ungulates
Funding
- Portuguese Foundation for Science and Technology (FCT)
- European Union (QREN)
- European Union (FEDER)
- European Union (COMPETE)
- QOPNA research unit [PEstC/QUI/UI0062/2013, FCOMP-01-0124-FEDER-037296]
- RNEM (Portuguese Network Mass Spectrometry)
- Genomics Applied To Genetic Resources
- North Portugal Regional Operational Programme (ON.2 - O Novo Norte)
- FCT Investigator grant [IF/01396/2013]
- [EXPL/BBB-BEP/0317/2012]
- [FCOMP-01-0124-FEDER-027554]
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Despite the importance of saliva in the regulation of oral cavity homeostasis, few studies have been conducted to quantitatively compare the saliva of different mammal species. Aiming to define a proteome signature of mammals' saliva, an in-depth SDS-PAGE-LC coupled to MS/MS (GeLC-MS/MS) approach was used to characterize the saliva from primates (human), carnivores (dog), glires (rat and rabbit), and ungulates (sheep, cattle, horse). Despite the high variability in the number of distinct proteins identified per species, most protein families were shared by the mammals studied with the exception of cattle and horse. Alpha-amylase is an example that seems to reflect the natural selection related to digestion efficacy and food recognition. Casein protein family was identified in all species but human, suggesting an alternative to statherin in the protection of hard tissues. Overall, data suggest that different proteins might assure a similar role in the regulation of oral cavity homeostasis, potentially explaining the specific mammals' salivary proteome signature. Moreover, some protein families were identified for the first time in the saliva of some species, the presence of proline-rich proteins in rabbit's saliva being a good example.
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