Journal
PROTEOMICS
Volume 15, Issue 15, Pages 2557-2559Publisher
WILEY
DOI: 10.1002/pmic.201500253
Keywords
Apicomplexa; Neospora; Proteogenomics; Protozoa; Toxoplasma
Funding
- NIAID NIH HHS [R01AI93220, R01 AI095094, R56 AI093220] Funding Source: Medline
Ask authors/readers for more resources
While high quality genomic sequence data is available for many pathogenic organisms, the corresponding gene annotations are often plagued with inaccuracies that can hinder research that utilizes such genomic data. Experimental validation of gene models is clearly crucial in improving such gene annotations; the field of proteogenomics is an emerging area of research wherein proteomic data is applied to testing and improving genetic models. Krishna etal. [Proteomics 2015, 15, 2618-2628] investigated whether incorporation of RNA-seq data into proteogenomics analyses can contribute significantly to validation studies of genome annotation, in two important parasitic organisms Toxoplasma gondii and Neospora caninum. They applied a systematic approach to combine new and previously published proteomics data from T. gondii and N. caninum with transcriptomics data, leading to substantially improved gene models for these organisms. This study illustrates the importance of incorporating experimental data from both proteomics and RNA-seq studies into routine genome annotation protocols.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available