4.6 Article

Effect of methylation on the side-chain pKa value of arginine

Journal

PROTEIN SCIENCE
Volume 25, Issue 2, Pages 479-486

Publisher

WILEY-BLACKWELL
DOI: 10.1002/pro.2838

Keywords

arginine; methylation; methyltransferase; pKa determination; pH titration; NMR spectroscopy

Funding

  1. Georgia Cancer Coalition (GCC), Georgia State University, Brain and Behavior Fellowship

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Arginine methylation is important in biological systems. Recent studies link the deregulation of protein arginine methyltransferases with certain cancers. To assess the impact of methylation on interaction with other biomolecules, the pK(a) values of methylated arginine variants were determined using NMR data. The pK(a) values of monomethylated, symmetrically dimethylated, and asymmetrically dimethylated arginine are similar to the unmodified arginine (14.2 +/- 0.4). Although the pK(a) value has not been significantly affected by methylation, consequences of methylation include changes in charge distribution and steric effects, suggesting alternative mechanisms for recognition.

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