TRANSMISSION OF MANNHEIMIA HAEMOLYTICA FROM DOMESTIC SHEEP (OVIS ARIES) TO BIGHORN SHEEP (OVIS CANADENSIS): UNEQUIVOCAL DEMONSTRATION WITH GREEN FLUORESCENT PROTEIN-TAGGED ORGANISMS

Previous studies demonstrated that bighorn sheep (Ovis canadensis) died Or pneumonia when commingled with domestic sheep (Ovis aries) but did not conclusively prove that the responsible pathogens were transmitted from domestic to bighorn sheep The objective of tins study was to determine. unambiguously, whether Mannheimia haemolytica can be transmitted from domestic to bighorn sheep when they commingle Four isolates of M haemolytica were obtained from the pharynx of two of four domestic sheep and tagged with a plasmid carrying the genes for green fluorescent protein (GFP) and ampicillin resistance (AP(R)) Four domestic sheep, colonized with the tagged bacteria, were kept about 10 m apart from four bighorn sheep for 1 mo with no clinical signs of pneumonia observed in the bighorn sheep during, that period The domestic and bighorn sheep were then allowed to have fence-line contact for 2 mo During that period, three bighorn sheep acquired the tagged bacteria from the domestic sheep At the end of the 2 mo of fence-line contact, the animals were allowed to commingle All lour bighorn sheep died 2 days to 9 days following commingling The lungs from all four bighorn sheep showed gross and histopathologic lesions characteristic of M haemolytica pneumonia Tagged M haemolytica were isolated from all four bighorn sheep, as confirmed by growth in ampicillin-containing culture medium. PCR-amplification of genes encoding GFP and Ap(R), and immunofluorescent staining of GFP These results unequivocally demonstrate transmission of M haemolytica from domestic to bighorn sheep, resulting in pneumonia and death of bighorn sheep.