Journal
PROTEIN SCIENCE
Volume 25, Issue 3, Pages 597-604Publisher
WILEY-BLACKWELL
DOI: 10.1002/pro.2853
Keywords
structure; dynamics; NMR; NF-B; IB alpha
Categories
Funding
- National Institutes of Health [GM71862]
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NF-B is a major transcription factor that mediates a number of cellular signaling pathways. Crystal structure analysis gives an incomplete picture of the behavior of the protein, particularly in the free state; free monomers or dimers of NF-B have never been crystallized. NMR analysis gives insights into the structure and dynamics of the protein in solution, but a necessary first step is the assignment of resonances. The size of the heterodimer of the Rel homology regions of the NF-B monomers p65 and p50 (72 kDa) prohibits the straightforward use of triple-resonance spectroscopy to obtain the assignments. However, the dynamic nature of the free heterodimer, in particular the independence of the DNA-binding and dimerization domains of each monomer, allows the assignments made on differentially labeled smaller domains to be mapped successfully onto the spectrum of the larger full-length RHR. Problematic areas such as the p65 nuclear localization sequence, which is disordered in the free protein, can be approached by residue-specific labeling and comparison with previously-published spectra of a short peptide with the same sequence. Overall, this NMR analysis of NF-B has given valuable insights into the highly dynamic nature of the free state, which is likely to play an important role in the functional cycle of NF-B in the cell.
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