4.6 Article

Highly Sensitive Real-Time In Vivo Imaging of an Influenza Reporter Virus Reveals Dynamics of Replication and Spread

Journal

JOURNAL OF VIROLOGY
Volume 87, Issue 24, Pages 13321-13329

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.02381-13

Keywords

-

Categories

Funding

  1. National Institute of General Medical Sciences [R00GM088484]
  2. Shaw Scientist Award
  3. Mirus Research Award
  4. Wisconsin Partnership Education and Research Committee New Investigator Program grant
  5. NIH National Research Service Award [T32 GM07215]

Ask authors/readers for more resources

The continual public health threat posed by the emergence of novel influenza viruses necessitates the ability to rapidly monitor infection and spread in experimental systems. To analyze real-time infection dynamics, we have created a replication-competent influenza reporter virus suitable for in vivo imaging. The reporter virus encodes the small and bright NanoLuc luciferase whose activity serves as an extremely sensitive readout of viral infection. This virus stably maintains the reporter construct and replicates in culture and in mice with near-native properties. Bioluminescent imaging of the reporter virus permits serial observations of viral load and dissemination in infected animals, even following clearance of a sublethal challenge. We further show that the reporter virus recapitulates known restrictions due to host range and antiviral treatment, suggesting that this technology can be applied to studying emerging influenza viruses and the impact of antiviral interventions on infections in vivo. These results describe a generalizable method to quickly determine the replication and pathogenicity potential of diverse influenza strains in animals.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available