Journal
JOURNAL OF VIROLOGY
Volume 87, Issue 11, Pages 6521-6525Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.00006-13
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Funding
- NIH [AI043222]
- Martin Delaney CARE Collaboratory
- Foundation for AIDS Research (amFAR)
- Johns Hopkins Center for AIDS Research
- Howard Hughes Medical Institute
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Current assays for quantification of HIV-1 virions rely on real-time reverse transcriptase (RT)-PCR detection of conserved regions of HIV-1 RNA and can be limited by detection of contaminating viral or plasmid DNA. We developed a novel RT-PCR assay using a reverse primer that hybridizes with the poly(A) tail of HIV-1 mRNAs, anchored by conserved viral nucleotides at the most distal region of the transcript. This assay can detect and quantify HIV-1 RNA with high specificity and sensitivity.
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