Journal
JOURNAL OF VIROLOGY
Volume 86, Issue 20, Pages 11128-11137Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.01700-12
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Funding
- Global COE Program (Frontier Biomedical Science Underlying Organelle Network Biology) of the Ministry of Education, Culture, Sports, Science & Technology of Japan
- Takeda Science Foundation
- Ministry of Science and Innovation of Spain [BIO2010-16705]
- U.S. National Institutes of Health [2P01AI060699-06A1, W000306844]
- [22790431]
- Grants-in-Aid for Scientific Research [24790439] Funding Source: KAKEN
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Severe acute respiratory syndrome (SARS) coronavirus (SCoV) is an enveloped virus containing a single-stranded, positive-sense RNA genome. Nine mRNAs carrying a set of common 5' and 3' untranslated regions (UTR) are synthesized from the incoming viral genomic RNA in cells infected with SCoV. A nonstructural SCoV nsp1 protein causes a severe translational shutoff by binding to the 40S ribosomal subunits. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5' UTR of host mRNA. However, the mechanism by which SCoV viral proteins are efficiently produced in infected cells in which host protein synthesis is impaired by nsp1 is unknown. In this study, we investigated the role of the viral UTRs in evasion of the nsp1-mediated shutoff. Luciferase activities were significantly suppressed in cells expressing nsp1 together with the mRNA carrying a luciferase gene, while nsp1 failed to suppress luciferase activities of the mRNA flanked by the 5' UTR of SCoV. An RNA-protein binding assay and RNA decay assay revealed that nsp1 bound to stem-loop 1 (SL1) in the 5' UTR of SCoV RNA and that the specific interaction with nsp1 stabilized the mRNA carrying SL1. Furthermore, experiments using an SCoV replicon system showed that the specific interaction enhanced the SCoV replication. The specific interaction of nsp1 with SL1 is an important strategy to facilitate efficient viral gene expression in infected cells, in which nsp1 suppresses host gene expression. Our data indicate a novel mechanism of viral gene expression control by nsp1 and give new insight into understanding the pathogenesis of SARS.
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