Journal
JOURNAL OF VIROLOGY
Volume 86, Issue 16, Pages 8750-8764Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.07224-11
Keywords
-
Categories
Funding
- NIH [R00-GM080352, T32-GM007750]
- UAB CFAR through the CNIHR program for new HIV investigators [P30-AI027767]
- National Center for Research Resources (NCRR), a component of the National Institutes of Health [P41-RR001209]
- Washington National Primate Research Center [P01-RR000166]
- [R01-AI076170]
Ask authors/readers for more resources
The gp120 subunit of the HIV Env glycoprotein is responsible for receptor interactions leading to viral entry and is a primary target for neutralizing antibodies. Most structural studies have focused on the heavily truncated, deglycosylated gp120 core, leaving fundamental aspects of the glycoprotein that are responsible for immune evasion and receptor-induced activation unresolved. Here we investigate full-length, glycosylated HIV gp120 in unliganded and CD4-bound forms by using small-angle X-ray scattering to visualize global structural reorganization and hydrogen/deuterium exchange to track changes in local conformational dynamics. The studies revealed unliganded full-length gp120 to be considerably more dynamic, particularly at the CD4 binding site, than suggested by previous studies of the subunit core alone. The large V1/V2 loops, previously unmapped, are positioned to mask the coreceptor binding site in an orientation that recapitulates that observed in the Env trimer. CD4 binding shifts V1/V2 to unmask the coreceptor binding site and triggers profound dynamic changes in gp120 spanning from the binding site to the gp41-interactive face of gp120. These findings provide further insights on the structural basis of Env antigenicity and immunogenicity and of allosteric effects upon receptor binding.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available