Differential Requirements for HIV-1 Vif-Mediated APOBEC3G Degradation and RUNX1-Mediated Transcription by Core Binding Factor Beta

Core binding factor beta (CBF beta), a transcription regulator through RUNX binding, was recently reported critical for Vif function. Here, we mapped the primary functional domain important for Vif function to amino acids 15 to 126 of CBF beta. We also revealed that different lengths and regions are required for CBF beta to assist Vif or RUNX. The important interaction domains that are uniquely required for Vif but not RUNX function represent novel targets for the development of HIV inhibitors.