Journal
JOURNAL OF VIROLOGY
Volume 86, Issue 9, Pages 4883-4891Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.06586-11
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Funding
- National Basic Research Program (973) of China [2011CB504705, 2012CB518900]
- National Natural Science Foundation of China [30972185, 30901073]
- Chinese Academy of Sciences [KSCX2-YW-R-158]
- National Key Technologies Research and Development Program of China [2010BAD04B01]
- Beijing Municipal Natural Science Foundation [6102018]
- Innovative Research Group of the National Natural Science Foundation of China (NSFC) [81021003]
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The influenza A virus matrix 1 protein (M1) shuttles between the cytoplasm and the nucleus during the viral life cycle and plays an important role in the replication, assembly, and budding of viruses. Here, a leucine-rich nuclear export signal (NES) was identified specifically for the nuclear export of the M1 protein. The predicted NES, designated the Flu-A-M1 NES, is highly conserved among all sequences from the influenza A virus subtype, but no similar NES motifs are found in the M1 sequences of influenza B or C viruses. The biological function of the Flu-A-M1 NES was demonstrated by its ability to translocate an enhanced green fluorescent protein (EGFP)-NES fusion protein from the nucleus to the cytoplasm in transfected cells, compared to the even nuclear and cytoplasmic distribution of EGFP. The translocation of EGFP-NES from the nucleus to the cytoplasm was not inhibited by leptomycin B. NES mutations in M1 caused a nuclear retention of the protein and an increased nuclear accumulation of NEP during transfection. Indeed, as shown by rescued recombinant viruses, the mutation of the NES impaired the nuclear export of M1 and significantly reduced the virus titer compared to titers of wild-type viruses. The NES-defective M1 protein was retained in the nucleus during infection, accompanied by a lowered efficiency of the nuclear export of viral RNPs (vRNPs). In conclusion, M1 nuclear export was specifically dependent on the Flu-A-M1 NES and critical for influenza A virus replication.
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