Journal
JOURNAL OF VIROLOGY
Volume 87, Issue 1, Pages 489-502Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.02186-12
Keywords
-
Categories
Funding
- Ministry of Health, Labor, and Welfare
- Ministry of Education, Culture, Sports, Science, and Technology
- Osaka University Global Center of Excellence Program
- Grants-in-Aid for Scientific Research [11J03343, 23689028, 24890105, 25115511, 24390113, 23790503] Funding Source: KAKEN
Ask authors/readers for more resources
Stress granules (SGs) are cytoplasmic foci composed of stalled translation preinitiation complexes induced by environmental stress stimuli, including viral infection. Since viral propagation completely depends on the host translational machinery, many viruses have evolved to circumvent the induction of SGs or co-opt SG components. In this study, we found that expression of Japanese encephalitis virus (JEV) core protein inhibits SG formation. Caprin-1 was identified as a binding partner of the core protein by an affinity capture mass spectrometry analysis. Alanine scanning mutagenesis revealed that Lys(97) and Arg(98) in the alpha-helix of the JEV core protein play a crucial role in the interaction with Caprin-1. In cells infected with a mutant JEV in which Lys(97) and Arg(98) were replaced with alanines in the core protein, the inhibition of SG formation was abrogated, and viral propagation was impaired. Furthermore, the mutant JEV exhibited attenuated virulence in mice. These results suggest that the JEV core protein circumvents translational shutoff by inhibiting SG formation through an interaction with Caprin-1 and facilitates viral propagation in vitro and in vivo.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available