Journal
JOURNAL OF VIROLOGY
Volume 84, Issue 18, Pages 9059-9069Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.00593-10
Keywords
-
Categories
Funding
- National Institutes of Health, National Institute of Allergy and Infectious Diseases
Ask authors/readers for more resources
Hepatitis E virus genotype 1 strain Sar55 replicated in subcloned Caco-2 intestinal cells and Huh7 hepatoma cells that had been transfected with in vitro transcribed viral genomes, and hepatitis E virions were released into the culture medium of both cell lines. Virus egress from cells depended on open reading frame 3 (ORF3) protein, and a proline-rich sequence in ORF3 was important for egress from cultured cells and for infection of macaques. Both intracellular ORF3 protein accumulation and virus release occurred at the apical membrane of polarized Caco-2 cells. ORF3 protein and lipids were intimately associated with virus particles produced in either cell line; ORF2 epitopes were masked in these particles and could not be immunoprecipitated with anti-ORF2.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available