Journal
JOURNAL OF VIROLOGY
Volume 85, Issue 4, Pages 1871-1874Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.01663-10
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Funding
- NIH [R01AI0175208, P41RR002250]
- Robert Welch Foundation [Q1242]
- Core Research for Evolutional Science and Technology (CREST) of Japan Science and Technology Corporation
- NIH through MSTP [GM07330]
- NIH through Gulf Coast Consortia [T15LM007093]
- United Kingdom MRC
- MRC [MC_U130115834] Funding Source: UKRI
- Medical Research Council [MC_U130115834] Funding Source: researchfish
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Resolving the nonicosahedral components in large icosahedral viruses remains a technical challenge in structural virology. We have used the emerging technique of Zernike phase-contrast electron cryomicroscopy to enhance the image contrast of ice-embedded herpes simplex virus type 1 capsids. Image reconstruction enabled us to retrieve the structure of the unique portal vertex in the context of the icosahedral capsid and, for the first time, show the subunit organization of a portal in a virus infecting eukaryotes. Our map unequivocally resolves the 12-subunit portal situated beneath one of the pentameric vertices, thus removing uncertainty over the location and stoichiometry of the herpesvirus portal.
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