Journal
JOURNAL OF VIROLOGY
Volume 84, Issue 17, Pages 8721-8731Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.00144-10
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Funding
- Public Health Service research [AI045135]
- National Institute of Allergy and Infectious Diseases, National Institutes of Health
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West Nile virus (WNV) infection leads to rapid and sustained Ca2+ influx. This influx was observed with different strains of WNV and in different types of cells. Entry during virion endocytosis as well as through calcium channels contributed to the Ca2+ influx observed in WNV-infected cells. Ca2+ influx was not detected after infection with vesicular stomatitis virus (VSV) and occurred only through endocytosis in Sindbis virus-infected cells. Caspase 3 cleavage and activation of several kinases, including focal adhesion kinase (FAK), mitogen-activated extracellular signal-regulated protein kinase (ERK1/2), and protein-serine kinase B alpha (Akt), at early times after WNV infection were shown to be dependent on Ca2+ influx. Although the activation of these kinases was sustained in virus-infected cells throughout infection, UV-inactivated WNV induced only a transient activation of FAK and ERK1/2 at early times after infection. The Ca2+-dependent FAK activation observed in WNV-infected cells was not mediated by alpha v beta 3 integrins. Reduction of Ca2+ influx at early times of infection by various treatments decreased the viral yield and delayed both the early transient caspase 3 cleavage and the activation of FAK, Akt, and ERK signaling. The results indicate that Ca2+ influx is required for early infection events needed for efficient viral replication, possibly for virus-induced rearrangement of the endoplasmic reticulum (ER) membrane. Increased caspase 3 cleavage at both early (transient) and late times of infection correlated with decreased activation of the FAK and ERK1/2 pathways, indicating a role for these kinases in extending the survival of flavivirus-infected cells.
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