Journal
JOURNAL OF VIROLOGY
Volume 84, Issue 21, Pages 11575-11579Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.00569-10
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Funding
- Netherlands Organization for Scientific Research (NWO-VIDI)
- Utrecht University (High Potential)
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The coronavirus nucleocapsid (N) protein is a virion structural protein. It also functions, however, in an unknown way in viral replication and localizes to the viral replication-transcription complexes (RTCs). Here we investigated, using recombinant murine coronaviruses expressing green fluorescent protein (GFP)-tagged versions of the N protein, the dynamics of its interactions with the RTCs and the domain(s) involved. Using fluorescent recovery after photobleaching, we showed that the N protein, unlike the nonstructural protein 2, is dynamically associated with the RTCs. Recruitment of the N protein to the RTCs requires the C-terminal N2b domain, which interacts with other N proteins in an RNA-independent manner.
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