Journal
JOURNAL OF VIROLOGY
Volume 84, Issue 6, Pages 3106-3110Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.01958-09
Keywords
-
Categories
Funding
- National Institutes of Health [CA067358, RD1AI052051]
Ask authors/readers for more resources
By using immunofluorescence microscopy to observe and analyze freshly made HIV-1 virions adsorbed onto cells, we found that they are inherently highly infectious, rather than predominantly defective as previously suggested. Surprisingly, polycations enhance titers 20- to 30-fold by stabilizing adsorption and preventing a previously undescribed process of rapid dissociation, strongly implying that infectivity assays for many viruses are limited not only by inefficient virus diffusion onto cells but also by a postattachment race between entry and dissociation. This kinetic competition underlies inhibitory effects of CCR5 antagonists and explains why adaptive HIV-1 mutations overcome many cell entry limitations by accelerating entry.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available