Journal
JOURNAL OF VIROLOGY
Volume 82, Issue 12, Pages 5860-5868Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.00076-08
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- Intramural NIH HHS Funding Source: Medline
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The human nuclear envelope proteins emerin and lamina-associated polypeptide 2 alpha (LAP2 alpha) have been proposed to aid in the early replication steps of human immunodeficiency virus type 1 (HIV-1) and murine leukemia virus (MLV). However, whether these factors are essential for HIV-1 or MLV infection has been questioned. Prior studies in which conflicting results were obtained were highly dependent on RNA interference-mediated gene silencing. To shed light on these contradictory results, we examined whether HIV-1 or MLV could infect primary cells from mice deficient for emerin, LAP2 alpha, or both emerin and LAP2 alpha. We observed HIV-1 and MLV infectivity in mouse embryonic fibroblasts (MEFs) from emerin knockout, LAP2 alpha knockout, or emerin and LAP2 alpha double knockout mice to be comparable in infectivity to wild-type littermate-derived MEFs, indicating that both emerin and LAP2 alpha were dispensable for HIV-1 and MLV infection of dividing, primary mouse cells. Because emerin has been suggested to be important for infection of human macrophages by HIV-1, we also examined HIV-1 transduction of macrophages from wild-type mice or knockout mice, but again we did not observe a difference in susceptibility. These findings prompted us to reexamine the role of human emerin in supporting HIV-1 and MLV infection. Notably, both viruses efficiently infected human cells expressing high levels of dominant-negative emerin. We thus conclude that emerin and LAP2 alpha are not required for the early replication of HIV-1 and MLV in mouse or human cells.
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