Journal
JOURNAL OF VIROLOGY
Volume 82, Issue 20, Pages 10308-10311Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.01204-08
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Funding
- National Institutes of Health [R01 33506, R01 33063, F32 NS056594, 2T32-GM007388-27, 5T32GM07312]
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Pseudorabies virus encodes a membrane protein (Us9) that is essential for the axonal sorting of virus particles within neurons and anterograde spread in the mammalian nervous system. Enhanced green fluorescent protein (GFP)-tagged Us9 mimicked the trafficking properties of the wild-type protein in nonneuronal cells. We constructed a pseudorabies virus strain that expressed Us9-GFP and tested its spread capabilities in the rat visual system and in primary neuronal cultures. We report that Us9-EGFP does not promote anterograde spread of infection and may disrupt packing of viral membrane proteins in lipid rafts, an essential step for Us9-mediated axonal sorting.
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