Journal
APPLIED RADIATION AND ISOTOPES
Volume 102, Issue -, Pages 5-9Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.apradiso.2015.04.010
Keywords
Competitive binding assay; Phosphorus-32; Radioligand; Sphingosine 1-phosphate; Sphingosine 1-phosphate Receptors; Sphingosine kinase 1
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Funding
- Department of Energy [DESC0008432]
- National Institute of Neurological Disorders and Stroke (NINDS) [R01NS075527]
- National Institute of Mental Health (NIMH) of the National Institutes of Health [MH092797]
- NATIONAL INSTITUTE OF MENTAL HEALTH [R33MH092797, R21MH092797] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R21NS061025, R01NS075527] Funding Source: NIH RePORTER
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Sphingosine-1-phosphate receptors (S1PRs) are important regulators of vascular permeability, inflammation, angiogenesis and vascular maturation. Identifying a specific S1PR PET radioligand is imperative, but it is hindered by the complexity and variability of current for binding affinity measurement procedures. Herein, we report a streamlined protocol for radiosynthesis of [P-32]S1P with good radiochemical yield (36-50%) and high radiochemical purity (>99%). We also report a reproducible procedure for determining the binding affinity for compounds targeting S1PRs in vitro. (C) 2015 Elsevier Ltd. All rights reserved.
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