Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 207, Issue -, Pages 60-65Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2014.06.004
Keywords
Encephalomyocarditis virus (EMCV); Real-time; RT-PCR; TaqMan; Detection
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Encephalomyocarditis virus (EMCV) is one of the major zoonosis pathogens and can cause acute myocarditis in young pigs or reproductive failure in sows. In this study, a TaqMan-based real-time reverse transcription polymerase chain reaction (RT-PCR) assay targeting 3D gene of EMCV was developed and their sensitivities and specificities were investigated. The results indicated that the standard curve had a wide dynamic range (10(1)-10(6) copies/mu L) with a linear correlation (R-2) of 0.996 between the cycle threshold (Ct) value and template concentration. The real-time RT-PCR assay is highly sensitive and able to detect 1.4 x 10(2) copies/mu L of EMCV RNA, as no cross-reaction was observed with other viruses. These data suggested that the real-time RT-PCR assay developed in this study will be suitable for future surveillance and specific diagnosis of EMCV-infection. (C) 2014 Elsevier B.V. All rights reserved.
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