Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 195, Issue -, Pages 164-169Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2013.08.029
Keywords
HIV; Reporter virus; Fusion; LTR; Post-entry event efficiency; Outcome
Funding
- Case Western Reserve University/University Hospitals Centers for AIDS Research: NIH [P30 AI036219]
- Foundation for AIDS Research (amfAR) [108257-51-RGRL]
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Fusion between the viral membrane of human immunodeficiency virus (HIV) and the host cell marks the end of the HIV entry process and the beginning of a series of post-entry events including uncoating, reverse transcription, integration, and viral gene expression. The efficiency of post-entry events can be modulated by cellular factors including viral restriction factors and can lead to several distinct outcomes: productive, latent, or abortive infection. Understanding host and viral proteins impacting post-entry event efficiency and viral outcome is critical for strategies to reduce HIV infectivity and to optimize transduction of HIV-based gene therapy vectors. Here, we report a combination reporter virus system measuring both membrane fusion and viral promoter-driven gene expression. This system enables precise determination of unstimulated primary CD4+ T cell subsets targeted by RIV, the efficiency of post-entry viral events, and viral outcome and is compatible with high-throughput screening and cell-sorting methods. (C) 2013 Elsevier B.V. All rights reserved.
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