Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 195, Issue -, Pages 63-66Publisher
ELSEVIER
DOI: 10.1016/j.jviromet.2013.08.037
Keywords
CCYV; RT-LAMP; Detection
Funding
- National Nature Foundation of China [U12041968]
- Postdoctoral Science Foundation of China [2013M530338]
Ask authors/readers for more resources
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of Cucurbit chlorotic yellows virus (CCYV). In this procedure, a set of four primers matching a total of six sequences in the coat protein gene region of CCYV was synthesized for the RT-LAMP assay using total RNA extracted from CCYV-infected melon leaf tissues, and the optimum reaction temperature and assay time were determined. The sensitivity assay showed that the virus was detectable in RT-LAMP reactions at dilutions of 1 x 10(-11), which was 10(5) times more sensitive than the RT-PCR assay. The RT-LAMP assay for CCYV and Sweet potato chlorotic stunt virus (SPCSV) exhibited high specificity for CCYV. This simple and sensitive method has potential for detection of CCYV in samples collected in the field. (C) 2013 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available