Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 210, Issue -, Pages 7-14Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2014.09.003
Keywords
Influenza; Neuraminidase; Enzyme-linked lectin assay; Serology; Antibody
Funding
- intramural PanFlu funds (CBER)
- ORISE
- NOW VICI grant [91896613]
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Antibodies to neuraminidase (NA), the second most abundant surface protein on influenza virus, contribute toward protection against influenza. The traditional thiobarbituric acid (TBA) method to quantify NA inhibiting antibodies is cumbersome and not suitable for routine serology. An enzyme-linked lectin assay (ELLA) described by Lambre et al. (1990) is a practical alternative method for measuring NA inhibition (NI) titers. This report describes optimization of the ELLA for measuring NI titers in human sera against influenza A viruses, using H6N1 and H6N2 viruses as antigens. The optimized ELLA is subtype-specific and reproducible. While the titers measured by ELLA are somewhat greater than those measured by a miniaturized TBA method, seroconversion rates are the same, suggesting similarity in assay sensitivity under these optimized conditions. The ELLA described in this report provides a practical format for routine evaluation of human antibody responses to NA. Published by Elsevier B.V.
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