Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 199, Issue -, Pages 25-28Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2013.12.023
Keywords
Electrical impedance assay; Virucide efficacy; Infectious bursal disease virus; Cytopathic effect
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A major problem with the testing of virucidal efficacy using current protocols is that scoring of virus-induced cytopathic effect (CPE) is dependent on subjective visual interpretation using light microscopy. The current report details the use of an electrical impedance assay (xCELLigence, ACEA Biosciences) for its utility in virucidal efficacy testing. In this study, the xCELLigence system was used in a procedure developed from guidelines given by the Deutsche Vereiniging zur Bekampfung der Viruskrankheiten (DVV) (German Association for the Control of Virus Diseases) in order to demonstrate the inactivation of infectious bursal disease virus using a commercial virucide. Although the modified DVV assay using the xCELLigence system yielded identical results (i.e. a 5-log 10 reduction in viral infectivity) as the traditional DVV assay, the system allows virucidal efficacy and cytotoxicity to be measured in a more precise and reproducible fashion. (C) 2014 Elsevier B.V. All rights reserved.
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