Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 187, Issue 1, Pages 177-181Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2012.09.016
Keywords
Ring trial; Rift Valley fever virus; ELISA; Virus-neutralization test; Europe
Funding
- EPIZONE network of Excellence [FOOD-CT-2006-016236]
- Biotechnology and Biological Sciences Research Council [BB/H009485/1] Funding Source: researchfish
- BBSRC [BB/H009485/1] Funding Source: UKRI
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A ring trial was organized to evaluate Rift Valley fever virus (RVFV) ELISAs by European laboratories. A total of five ELISAs, two of which specific for IgM antibodies, were evaluated by six participants. Sera were derived from cattle or sheep and originated from either a RVFV endemic area, a RVFV-free area or from experimental infection studies. Cohen's kappa analysis showed higher than 90% agreement of two commercially available ELISAs with the virus neutralization test, suggesting that primary screening as well as serological confirmation using these ELISAs is feasible. More extensive validations with sera of known IgM status are, however, required to determine agreement between IgM ELISAs. (C) 2012 Elsevier B.V. All rights reserved.
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