Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 189, Issue 2, Pages 393-396Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2013.03.008
Keywords
Hepatitis B virus; Bio-nanocapsule; His-tag; Affinity chromatography
Funding
- Special Coordination Fund for Promoting Science and Technology, Creation of Innovative Centers for Advanced Interdisciplinary Research Areas (Innovative Bioproduction Kobe) from the Ministry of Education, Culture, Sports and Technology (MEXT)
- Science Research Grants from the Ministry of Health, Labour and Welfare, Japan
Ask authors/readers for more resources
A bio-nanocapsule derived from the hepatitis B virus (HBV) is expected to be useful as a drug delivery system carrier. Because various types of bio-nanocapsules have been developed, a simple and versatile purification method for bio-nanocapsules would be useful. Therefore, this study was focused on establishing a simple purification method using affinity chromatography by inserting a histidine tag (His-tag) into a bio-nanocapsule. The method achieved a simple, one-step purification with a yield that was 2.5-fold higher than conventional ultracentrifugation, and thus would be a desirable alternative method for recombinant virus-like particle purification. Crown Copyright (c) 2013 Published by Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available