Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 191, Issue 2, Pages 148-154Publisher
ELSEVIER
DOI: 10.1016/j.jviromet.2012.07.015
Keywords
Diagnosis; Loop-mediated isothermal amplification; Cassava brown streak disease; CBSV; UCBSV
Funding
- Catholic Relief Services
- Bill and Melinda Gates Foundation
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The causal agents of cassava brown streak disease have recently been identified as Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). Primers have been developed for rapid detection of these viruses by reverse transcription loop-mediated isothermal amplification (RT-LAMP). Performance of the RT-LAMP assays compared favourably with published RT-PCR and real-time RT-PCR methods. Furthermore, amplification by RT-LAMP is completed in 40 mm and does not require thermal cycling equipment. Modification of the RT-LAMP reactions to use labelled primers allowed rapid detection of amplification products using lateral flow devices containing antibodies specific to the incorporated labels, avoiding the need for fluorescence detection or gel electrophoresis. Crown Copyright (C) 2012 Published by Elsevier B.V. All rights reserved.
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