Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 177, Issue 2, Pages 168-173Publisher
ELSEVIER
DOI: 10.1016/j.jviromet.2011.08.002
Keywords
Dengue virus; One step real time multiplex RT-PCR; Validation
Funding
- Wellcome Trust [084368] Funding Source: Medline
Ask authors/readers for more resources
Dengue is mosquito-borne virus infection that annually causes similar to 50 million clinically apparent cases worldwide. An internally controlled one-step real-time multiplex RT-PCR assay was developed for detection and quantitation of DENV RNA in plasma sample by using specific primers and fluorogenic TaqMan probes. All primers and probes targeted sequences near the 3' end of the NS5 gene. The method comprised two multiplex assays and was validated for sensitivity, specificity, linearity, reproducibility and precision. An internal control template was spiked into each clinical specimen to provide quality assurance for each experimental step. The assay allowed for detection of between 0.5 and 3 infectious particles per mL, is rapid and has been operationally characterized in 287 Vietnamese dengue patients from two therapeutic intervention trials at the Hospital for Tropical Diseases, Ho Chi Minh City, Viet Nam. (C) 2011 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available