Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 175, Issue 1, Pages 28-37Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2011.04.011
Keywords
Lactococcus lactis; Gene expression; Rotavirus; Vaccine
Funding
- Agencia Nacional de Promocion Cientifica y Tecnologica (ANPCyT, Argentina)
- Consejo Nacional de investigaciones Cientificas y Tecnologicas (CONICET, Argentina)
- Universidad Nacional de Rosario (UNR)
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Rotaviruses are the major cause of worldwide infectious diarrhea in children and vaccination is considered to be the most effective way to control these infections. The development of a mucosal live vaccine using the food-grade lactic acid bacteria Lactococcus lactis as antigen vehicle is an attractive and safe vaccination strategy against rotavirus. In this study, the construction of recombinant L lactis strains able to produce the rotavirus spike-protein subunit VP8* in cytoplasmic. secreted and cell wall-anchored forms is reported. Evaluation of the immune response generated after immunization was conducted in a mouse model. The present study shows that animals inoculated orally with the L lactis strain producing the cytoplasmic form of VP8* (LL1) developed significant levels of intestinal IgA antibodies while animals receiving L lactis producing the cell wall-anchored VP8* form (LL3) exhibited anti-VP8* antibodies at both intestinal and systemic levels. Furthermore, it was observed that intestinal antibodies of the LL1-treated group and serum antibodies of the LL3-treated group were able to block rotavirus infection by 50% and 100%, respectively. These encouraging results represent a step towards the development of a new and safe mucosal vaccine against rotavirus. (C) 2011 Elsevier B.V. All rights reserved.
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