Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 171, Issue 1, Pages 292-294Publisher
ELSEVIER
DOI: 10.1016/j.jviromet.2010.11.002
Keywords
Maize chlorotic mottle virus; Real-time RT-PCR; Quarantine virus
Funding
- Key Project of the National Science & Technology Pillar Program [2006BAK10B06, 2006BAD08A13, 2006BAD08A16-1]
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Maize chlorotic mottle virus (MCMV) causes corn lethal necrosis disease, and can be transmitted through infected maize seeds. It remains a challenge to detect this virus in the seeds to prevent its introduction and infection. For this purpose, a real-time TaqMan RT-PCR procedure for efficient detection of MCMV was developed. The sensitivity of the method was 4 fg of total RNA or 25 copies of RNA transcripts, which was approximately ten-fold higher than conventional RT-PCR gel electrophoresis method. The successful detection of MCMV in maize seeds suggested the feasibility of this procedure for routine testing. (C) 2010 Elsevier B.V. All rights reserved.
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