A new method for detection of pandemic influenza virus using High Resolution Melting analysis of the neuraminidase gene

Diagnostic methods based upon exclusive detection of haemagglutinin do not detect sequence variation in other gene segments of the Influenza A virus. A complementary approach is described based upon high-resolution melting curve analysis of the neuraminidase gene, an approach with the potential ability to detect small changes in the neuraminidase sequence without the need for specific probes. (C) 2010 Elsevier B.V. All rights reserved.