Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 165, Issue 2, Pages 268-276Publisher
ELSEVIER
DOI: 10.1016/j.jviromet.2010.02.007
Keywords
Hepatitis C virus; Replication complex; NS5A; Enhanced green fluorescent protein; Inducible cell line; High-content screening
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The hepatitis C virus (HCV) replicates its genome on a membrane-associated replication complex. These complexes are represented by dot-like structures on the endoplasmic reticulum when standard fluorescence microscopy techniques are applied. To screen compound libraries for inhibitors interfering with the formation of the HCV replication complex independent of RNA replication, an image-based high-content screening assay was developed utilizing inducible expression of the HCV non-structural proteins NS3-5B in an U2-OS Tet-On cell line. An eGFP was fused to NS5A for the detection of replication complexes. The cell line was tightly regulated and the eGFP insertion within NS5A did not alter polyprotein processing. The NS5AeGFP signal colocalized with other non-structural proteins in dot-like structures. Accompanying image analysis tools were developed enabling the detection of changes in replication complex formation. Finally, the addition of a HCV NS3/4A protease inhibitor resulted in a dose-dependent reduction of dot-like structures demonstrating the practicability of the assay. (C) 2010 Elsevier By. All rights reserved.
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