Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 161, Issue 1, Pages 91-97Publisher
ELSEVIER
DOI: 10.1016/j.jviromet.2009.05.023
Keywords
Random PCR screening; Measles patients; Identification of unknown pathogens; Type 1 human herpes simplex virus
Funding
- National Basic Research Priorities Programme [2005CB522902]
- National Mega Project for Infectious Diseases [2009ZX10602-11]
- National Natural Science [30425041]
- Fudan University [EYF162001]
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Several viral diseases exhibit measles-like symptoms. Differentiation of suspected cases of measles with molecular epidemiological techniques in the laboratory is useful for measles surveillance. In this study, a random PCR screening system was undertaken for the identification of isolates from patients with measles-like symptoms who exhibited cytopathic effects, but who had negative results for measles virus-specific reverse transcription (RT)-PCR and indirect immunofluorescence assays. Sequence analysis of random amplified PCR products showed that they were highly homologous to type 1 human herpes simplex virus (HSV-1). The results were further confirmed by an HSV-1-specific TaqMan real-time PCR assay. The random PCR screening system described in this study provides an efficient procedure for the identification of unknown viral pathogens. Measles-like symptoms can also be caused by HSV-1, suggesting the need to include HSV-1 in differential diagnoses of measles-like diseases. (C) 2009 Elsevier B.V. All rights reserved.
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