Journal
JOURNAL OF VETERINARY MEDICAL SCIENCE
Volume 73, Issue 11, Pages 1445-1451Publisher
JAPAN SOC VET SCI
DOI: 10.1292/jvms.11-0282
Keywords
antisense RNA; fowl cholera; noncapsulated strain; Pasteurella multocida
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Funding
- Faculty of Veterinary Medicine, Chiang Mai University
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A mutant strain, PBA322, was constructed by electroporation of a phagemid containing the coding region of antisense RNA of the ompH gene, encoding 39 kDa capsular protein or OmpH, into the parental strain P-I 059 (serovar A:3) of Pasteurella multocida, and the pathogenicity was determined in mice and chickens. Grayish colonies of the mutant, indicating loss of capsule synthesis, were observed under a stereomicroseope using obliquely transmitted light, while iridescent colonies were observed for the parental strain. Moreover, strain PBA322 showed a low amount of OmpH compared with the parental strain on SDS-PAGE. Additionally, the capsule of strain PBA322 was thinner than that of the parental strain according to electron microscopy, correlating to the attenuation against chickens. In conclusion, strain PBA322, the mutant of P. multocida strain P-1059, was completely attenuated for chickens.
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