Journal
JOURNAL OF VETERINARY MEDICAL SCIENCE
Volume 71, Issue 8, Pages 1009-1015Publisher
JAPAN SOC VET SCI
DOI: 10.1292/jvms.71.1009
Keywords
Borna disease virus; epidemiology; raccoon
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We have examined the seroprevalence of BDV in wild Raccoons (Procyon lotor) in Hokkaido, Japan. Serum samples from raccoons were examined using ELISA and Western blot assays to detect the presence of serum antibodies that react specifically to BDV antigens. Among 549 investigated individuals, eleven (2.0%) showed a positive reaction to BDV antigens. Brain tissue samples from five individuals were subjected to RT-PCR, which detected BDV sequences in three of them. Sequence analysis revealed a high degree of genetic conservation between BDV sequences derived from raccoons and previously published sequences derived from other animal species.
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