Journal
JOURNAL OF VETERINARY MEDICAL SCIENCE
Volume 71, Issue 2, Pages 239-241Publisher
JAPAN SOC VET SCI
DOI: 10.1292/jvms.71.239
Keywords
Forage Rice; HPLC; Silage; Ustiloxin A
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Funding
- Integrated Research Project for Developing a Japanese-style Forage Feeding System to Increase
- Ministry of Agriculture, Forestry and Fisheries of Japan
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We describe a high-performance liquid chromatographic method for determining ustiloxin A, a mycotoxin produced by Ustilaginoidea virens, in forage rice silage. Lyophilized silage samples were ground and extracted with water. The extracts were purified by solid-phase extraction and subjected to high-performance liquid chromatography using an octadecylsilane-bonded column. Separated ustiloxin A was detected with ultraviolet (UV) absorption at 254 nm. The limit of quantitation for ustiloxin A in silage found to be 2.5 mg/kg. The present method can be Used for routine monitoring of the contamination of ustiloxin A in forage rice silage.
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