Journal
PROCESS BIOCHEMISTRY
Volume 50, Issue 2, Pages 279-284Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2014.11.009
Keywords
Plant cell culture; Paclitaxel; Microwave-assisted extraction (MAE); Acid hydrolysis; Simultaneous process
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Funding
- National Research Foundation of Korea (NRF) - Korean government (MEST) [2011-0010907]
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In this study, a microwave-assisted extraction (MAE)/acid hydrolysis simultaneous process was developed for recovering the anticancer agent paclitaxel from plant cell cultures of Taxus chinensis. The optimal pH of the extraction solution (90% aqueous methanol) for hydrolysis was 2.2 at fixed extraction time (6 min), ratio of extraction solution to biomass (1:1, v/w), and extraction temperature (40 degrees C). In the MAE/acid hydrolysis simultaneous process, the paclitaxel recovery was 2.2-fold higher than in the existing extraction methods. Regarding changes in the content of glycoside (7-xylosyl paclitaxel as sugar-binding paclitaxel) and paclitaxel depending on the inclusion of acid hydrolysis in the MAE process, the content of 7-xylosyl paclitaxel decreased after acid hydrolysis whereas the content of paclitaxel increased. Based on this result, it was confirmed that acid hydrolysis breaks down a glycosidic bond of glycoside (sugar-binding paclitaxel), and so the recovery of paclitaxel increases. (C) 2014 Elsevier Ltd. All rights reserved.
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