Journal
PROCESS BIOCHEMISTRY
Volume 50, Issue 5, Pages 746-751Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2015.02.006
Keywords
Enzyme ratios; Chloroperoxidase-glucose oxidase; Thioanisole oxidation; Bi-enzymatic oxidation
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The optimum application of chloroperoxidase from Caldariomyces fumago in oxidations with hydrogen peroxide depends on the mode of addition of the oxidant:The use of the previously reported combination of chloroperoxidase and glucose oxidase, for in situ generation of hydrogen peroxide, was studied in more detail using thioanisole as a model substrate. Maximum yields and enantiopurities were observed at high chloroperoxidase reaction rates and not at low hydrogen peroxide formation rates, as would be expected considering the instability of CPO at high hydrogen peroxide concentrations. Glucose oxidase catalyzed aerobic sulfoxidation, affording racemic sulfoxide, was observed as an unexpected and novel side-reaction. It was attributed to oxidation by a flavin hydroperoxide formed by reaction of the free flavin cofactor associated with glucose oxidase with dioxygen. The rate of this side-reaction depended on the amount of co-solvent in the system and the enantiopurity of the oxidation product could thus be improved by lowering the co-solvent concentration. (C) 2015 Elsevier Ltd. All rights reserved.
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