Journal
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
Volume 24, Issue 5, Pages 954-958Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/1040638712452732
Keywords
Avian Influenza A virus; chicken sera; hemagglutination inhibition test; H5N1; nonspecific inhibitors
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The hemagglutination inhibition (HI) assay is the standard diagnostic test for detection of antibodies to avian influenza viruses. It is well known that chicken serum does not require additional serum pretreatment to remove nonspecific inhibitors (NSIs). However, NSIs were recognized in certain Korean local breeds. In the present study, various treatments were compared to remove such NSIs. Heat treatment, red blood cell adsorption, and kaolin treatment did not remove NSIs effectively, and treatment with periodate only partly eliminated the NSIs. Receptor destroying enzyme (RDE) treatment appeared to effectively remove NSIs from chicken sera, regardless of breeds. It is proposed that RDE treatment should be included in the HI tests for serological diagnosis of avian Influenza A virus.
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