Journal
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
Volume 20, Issue 1, Pages 68-71APublisher
AMER ASSOC VETERINARY LABORATORY DIAGNOSTICIANS INC
DOI: 10.1177/104063870802000113
Keywords
Blood smear; GM1 gangliosidosis; Japan; molecular screening; Shiba dogs
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Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) [14560258, 16380210]
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Molecular screening of GM1 gangliosidosis in Shiba dogs was carried out in northern Japan using blood smear specimens after prolonged storage. Of 125 specimens obtained from 3 veterinary teaching hospitals for this screening, 68 specimens (54%) were adequate for direct amplification in a polymerase chain reaction (PCR)-based DNA test, and the percentage of adequacy was different at each hospital (34%, 73%, and 100%), suggesting that the amount of blood on the smear and the storage condition of specimens may affect adequacy. Of the 68 dogs examined, 2 dogs (2.9%) were heterozygous carriers for this disease and the other dogs were all genotypically normal. The results suggest blood smear specimens can be useful for PCR testing after prolonged storage provided specimens contain a generous amount of blood and have been adequately stored. The study also suggests that GM 1 gangliosidosis may be widely prevalent in the Shiba dog population in northern Japan.
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