Journal
JOURNAL OF VASCULAR SURGERY
Volume 56, Issue 4, Pages 1078-1088Publisher
MOSBY-ELSEVIER
DOI: 10.1016/j.jvs.2012.03.002
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Funding
- USA National Institutes of Health [R01 GM 49039]
- Pervasis Therapeutics
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Objective: High restenosis rates are a limitation of peripheral vascular interventions. Previous studies have shown that surgical implantation of a tissue-engineered endothelium onto the adventitia surface of injured vessels regulates vascular repair. In the present study, we developed a particulate formulation of tissue-engineered endothelium and a method to deliver the formulation perivascular to injured blood vessels using a percutaneous, minimally invasive technique. Methods: Stainless steel stents were implanted in 18 balloon-injured femoral arteries of nine domestic swine, followed by ultrasound-guided percutaneous perivascular injection of gelatin particles containing cultured allogeneic porcine aortic endothelial cells (PAE). Controls received injections of empty particles (matrix) or no perivascular injection (sham) after stent deployment. Animals were sacrificed after 90 days. Results: Angiographic analysis revealed a significantly greater lumen diameter in the stented segments of arteries treated with PAE/matrix (4.72 +/- 0.12 mm) compared with matrix (4.01 +/- 0.20 mm) or sham (4.03 +/- 0.16 mm) controls (P < .05). Similarly, histologic analysis revealed that PAE/matrix-treated arteries had the greatest lumen area (20.4 +/- 0.7 mm(2); P < .05) compared with controls (16.1 +/- 0.9mm(2) and 17.1 +/- 1.0 mm(2) for sham and matrix controls, respectively) and the smallest intimal area (3.3 +/- 0.4 mm(2); P < .05) compared with controls (6.2 +/- 0.5 mm(2) and 4.4 +/- 0.5 mm(2) for sham and matrix controls, respectively). Overall, PAE-treated arteries had a 33% to 50% decrease in percent occlusion (P < .05) compared with controls. Histopathological analysis revealed fewer leukocytes present in the intima in the PAE/matrix group compared with control groups, suggesting that the biological effects were in part due to inhibition of the inflammatory phase of the vascular response to injury. Conclusions: Minimally invasive, perivascular delivery of PAE/matrix to stented arteries was performed safely using ultrasound-guided percutaneous injections and significantly decreased stenosis. Application at the time of or subsequent to peripheral interventions may decrease clinical restenosis rates. (J Vasc Surg 2012;56:1078-88.)
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